L-methionine γ-lyase from Thermo-tolerant fungi: Isolation, Identification of the potent producers, and Statistical Optimization of Production via Response surface methodology

In the current study, thermo-tolerant fungal isolates were screened for their ability to metabolize L-methionine by γ-lyase (MGL). Among 63 isolates, fifteen exhibited MGL induction as evidenced qualitative assay method using phenol red. Then, quantitative indicates that coded 26 & 37 most potent production. Fungal mycelium treatment under sonication process proved isolate No. could be release with maximum activity, meanwhile has been produced extracellularly same culture conditions. Thereafter, characterization and identification of these two was carried out morphological, microscopic examination molecular techniques. consequence this characterization, identified Aspergillus fumigates Rhizomucor miehei respectively. Different conditions Plakket-Burmn design define significant parameters affect strains. Using response surface model, independent optimized through a central composite (CCD) maximize extracellular intracellular A. fumigatus R. miehei. Statistical optimization Response methodology (RSM) revealed yeast extract, incubation period temperature factors production where 8.27 U/mg. Additionally, glycerol, PH, inoculum size efficient 12.37